间接ELISA和竞争ELISA检测小反刍兽疫病毒抗体的比较

doi:10.11843/j.issn.0366-6964.2014.02.024

Abstract

Abstract:

The indirect ELISA and the competitive ELISA method were compared in the detection of serum antibody against Peste des Petits Ruminants Virus (PPRV).The PPRV N protein to be used as a coating antigen was expressed in BL21 and purified by Ni Sepharose FF column.Ninety-four sheep and twenty-five goats serum samples were detected by the ELISA methods based on the recombinant N protein.The coincidence rate of the indirect ELISA and the competitive ELISA kit was 74.8%，the coincidence rate of the competitive ELISA and the competitive ELISA kit was 96.6%.The detection results showed that the competitive ELISA is more reliable than the indirect ELISA method， and the competitive ELISA is suitable for clinical application.

CLC Number:

S852.659.5

WANG Jing，LI Gang，SHI Li-jun. Comparison of Indirect ELISA and Competitive ELISA for Detecting Peste des Petits Ruminants Virus Antibody[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(2): 333-336.

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Comparison of Indirect ELISA and Competitive ELISA for Detecting Peste des Petits Ruminants Virus Antibody

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